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While extremely sensitive to atomic-scale features, AFMs also are extremely sensitive to interference from acoustic noise, temperature shifts and vibration, among other factors. This makes it difficult or impossible either to hold the probe in one place to observe the specimen under it over time (useful for studying the dynamics of proteins) or to move the probe away and return to exactly the same spot (potentially useful for nanoscale manufacturing). "At this scale, it's like trying to hold a pen and draw on a sheet of paper while riding in a jeep," observes NIST physicist Thomas Perkins. A few instruments in specialized labs, including some at NIST, solve this problem by operating at extremely cold temperatures in ultra-high vacuums and in heavily isolated environments, but those options aren't available for the vast majority of AFMs, particularly those used in bioscience laboratories where the specimen often must be immersed in a fluid.
In its most recent work, the JILA team has controlled the probe's position in three dimensions to better than 40 picometers (1 nanometer = 1000 picometers) over 100 seconds. In imaging applications, they showed the long-term drift at room temperature was a mere 5 picometers per minute, a 100-fold improvement over the best previous results under ambient conditions. Just like photographers use the stability of a tripod and longer exposures to improve picture quality, the JILA team used their improved stability to scan the AFM probe more slowly, leading to a 5-fold improvement in AFM image quality. A bonus, says Perkins, is the technique works with standard commercial probes.
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